other:
Recombinant:Article Title: Endogenous p53 inhibitor TIRR dissociates systemic metabolic health from oncogenic activity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Abca1 Novus Biologicals Cat# NB400-105; RRID: AB_10000630 anti-p53 primary antibody Leica Cat#P53-CM5P-L RRID: AB_563933 anti-phospho-p53 (Ser15) antibody Cell Signaling Cat#9284 RRID: AB_331464 anti-acetyl-p53 (Lys379) antibody Cell Signaling Cat#2570 RRID: AB_823591 Anti-Tubulin Santa Cruz Biotechnology Cat# sc-9104 RRID: AB_2241191 antiCD40 (Clone HM40-3) Invitrogen Cat#14-0402-82 RRID: AB_467228 anti-B220 (PE Rat anti-mouse CD45R-B220 clone RA3-6B2 BD) Pharmingen Cat#561878 RRID: AB_10893353 anti-IgG1 (FITC Rat anti-mouse IgG1 BD) Pharmingen Cat#553443 RRID: AB_394862 Biological samples Human visceral adipose tissue biopsies This paper N/A Mouse tissues (perigonadal adipose tissue, liver, hypothalamus, spleen, quadriceps muscle) This paper N/A Chemicals, peptides, and recombinant proteins Standard chow PicoLab Mouse Diet 20 LabDiet Cat# 5058 HFD, 60% kcal fat Research Diets Inc. Cat#D12492 Dexa instrument Piximus GE N/A Lipofectamine-RNAiMax Thermo Fisher Scientific Cat#13778030 IL4 R&D systems Cat#404-ML-010 DMEM GIBCO Cat# 11965 3-isobutyl-1-methylxanthine (IBMX) Sigma-Aldrich Cat#I5879 dexamethasone Sigma-Aldrich Cat#D4902 rosiglitazone Sigma-Aldrich Cat#R2408 Opti-MEM Thermo Fisher Scientific Cat#31985070 Critical commercial assays Piccolo lipid panel plus Fisher Scientific Cat#07-P02-12A Ultrasensitive mouse insulin ELISA Crystal Chem, USA Cat#90080 Adiponectin ELISA Crystal Chem, USA Cat#80569 Leptin ELISA Crystal Chem, USA Cat#90030 Deposited data RNA-seq data This paper GSE210900 https://www.ncbi.nlm.nih. gov/geo/query/acc.cgi?acc=GSE210900 Raw data This paper Mendeley Data: http://www.doi.org/ 10.17632/yvhn4hn3b8.1 Original code This paper https://zenodo.org/records/6904028 Experimental models: Cell lines 3T3-L1 cells ATCC Cat#CL-173 TIRRfl/fl MEFs This paper N/A TIRRKO MEFs This paper N/A p53KO;TIRRKO MEFs This paper N/A Experimental models: Organisms/strains EIIaCre mice Jax stock #003724 CD19-Cre mice Jax stock #006785 Adp-Cre mice Jax stock #028020 (Continued on next page) Cell Reports 43, 114337, June 25, 2024 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Camk2a-Cre mice Jax stock #005359 p53KO mice Jax stock #002101 TIRRfl/fl mice This paper N/A TIRRKO mice This paper N/A p53HET;TIRRKO mice This paper N/A p53KO;TIRRKO mice This paper N/A AdipoQ-CreTG/WT;TIRRfl/fl mice This paper N/A Camk2a-CreTG/WT;TIRRfl/fl mice This paper N/A TIRRBcell KO mice This paper N/A Oligonucleotides See Table S2 for qPCR and ChIP-qPCR primers N/A N/A Software and algorithms Graphpad Prism Graphpad Software Inc., La Jolla, CA, USA https://www.graphpad.com Python, version 3.7.10 pandas, numpy, scipy, statsmodels, sklearn and umap libraries The Python Software Foundation (PSF) https://www.python.org ImajeJ NIH https://imagej.nih.gov/ij/
Article Title: Dual functions of NPC1L1 for cholesterol transport: Cholesterol capture and regulation of inner-leaflet cholesterol accessibility
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GFP rabbit IgG fraction Thermo Fisher Scientific Cat# A11122; RRID: AB_221569 Anti-Niemann-Pick type C1 Like-1 NOVUS Biologicals Cat# NB400-128; RRID: AB_10000815 Na, K-ATPase antibody Cell Signaling Technology Cat# 3010; RRID: AB_2060983 Anti-rabbit IgG Cytiva Cat# NA934; RRID: AB_772206 Chemicals, peptides, and recombinant proteins Phosphatidylcholine Nacalai Tesque Cat# 27554–01 Oleic acid Nacalai Tesque Cat# 25629–75 Monoolein Nacalai Tesque Cat# 23408–12 NaOH solution Nacalai Tesque Cat# 94611–45 HCl solution Nacalai Tesque Cat# 37313–25 [3H]cholesterol (cholesterol, [1, 2, 3H(N)]-) Perkin Elmer Cat# NET139 Lipoprotein deficient serum (LPDS) from fetal calf Sigma-Aldrich – Lysophosphatidylcholine Santa Cruz Biotechnology Cat# SC-473611 Sodium taurocholate FUJIFILM Wako Cat# 197–10033 Ezetimibe Biosynth Carbosynth Cat# FE23215 Imidazole FUJIFILM Wako Cat# 095–00015 NaH2PO3 FUJIFILM Wako Cat# 197–09705 Na2HPO3 FUJIFILM Wako Cat# 197–02865 Lysozyme FUJIFILM Wako Cat# 123–06721 NaCl Nacalai Tesque Cat# 31320–05 Glycerol Nacalai Tesque Cat# 17018–83 Isopropyl-β-thiogalactopyranoside (IPTG) Nacalai Tesque Cat# 19742–81 IMAC Ni-charged resin Bio-Rad Cat# 156–0135 Protease inhibitor cocktail Roche Cat# 05056489001 CBB Stain One Nacalai Tesque Cat# 04543–51 Paraformaldehyde FUJIFILM Wako Cat# 163–20145 VECTASHIELD Mounting Medium Vector Laboratories Cat# H-1000 Sodium dodecyl sulfate (SDS) Nacalai Tesque Cat# 31606–75 Tris (hydroxymethyl) aminomethane Nacalai Tesque Cat# 35406–91 IGEPAL CA-630 Sigma-Aldrich Cat# 18896 Sodium deoxycholate FUJIFILM Wako Cat# 192–08312 Page Ruler Prestatined Protein Ladder Thermo Fisher Scientific Cat# 26616 ECL Prime Western Blotting Detection Reagent Cytiva Cat# RPN2232 Experimental models: Cell lines McA-RH7777 cells ATCC CRL-1601 MDA-MB-231 cells ATCC HTB-26 Oligonucleotides siRNA targeting sequence: NPC1L1 #1: sense: 5′-GAGGCCUUCUUAGAGGAAA-3′ antisense: 5′-UUUCCUUCUAAGAAGGCCUC-3′ This paper N/A (Continued on next page) iScience 29, 117625, October 16, 2026 15 .. McA-RH7777 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Nacalai Tesque) supplemented with 10% fetal bovine serum (FBS) (Cosmo Bio, Tokyo, Japan), 2 mM L-glutamine (Nacalai Tesque), 1% MEM Non-Essential Amino Acid solution (NEAA) (Thermo Fisher Scientific, MA, USA), and 100 units/mL penicillin streptomycin (PCSM) (Nacalai Tesque).
Enzyme-linked Immunosorbent Assay:Article Title: Endogenous p53 inhibitor TIRR dissociates systemic metabolic health from oncogenic activity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Abca1 Novus Biologicals Cat# NB400-105; RRID: AB_10000630 anti-p53 primary antibody Leica Cat#P53-CM5P-L RRID: AB_563933 anti-phospho-p53 (Ser15) antibody Cell Signaling Cat#9284 RRID: AB_331464 anti-acetyl-p53 (Lys379) antibody Cell Signaling Cat#2570 RRID: AB_823591 Anti-Tubulin Santa Cruz Biotechnology Cat# sc-9104 RRID: AB_2241191 antiCD40 (Clone HM40-3) Invitrogen Cat#14-0402-82 RRID: AB_467228 anti-B220 (PE Rat anti-mouse CD45R-B220 clone RA3-6B2 BD) Pharmingen Cat#561878 RRID: AB_10893353 anti-IgG1 (FITC Rat anti-mouse IgG1 BD) Pharmingen Cat#553443 RRID: AB_394862 Biological samples Human visceral adipose tissue biopsies This paper N/A Mouse tissues (perigonadal adipose tissue, liver, hypothalamus, spleen, quadriceps muscle) This paper N/A Chemicals, peptides, and recombinant proteins Standard chow PicoLab Mouse Diet 20 LabDiet Cat# 5058 HFD, 60% kcal fat Research Diets Inc. Cat#D12492 Dexa instrument Piximus GE N/A Lipofectamine-RNAiMax Thermo Fisher Scientific Cat#13778030 IL4 R&D systems Cat#404-ML-010 DMEM GIBCO Cat# 11965 3-isobutyl-1-methylxanthine (IBMX) Sigma-Aldrich Cat#I5879 dexamethasone Sigma-Aldrich Cat#D4902 rosiglitazone Sigma-Aldrich Cat#R2408 Opti-MEM Thermo Fisher Scientific Cat#31985070 Critical commercial assays Piccolo lipid panel plus Fisher Scientific Cat#07-P02-12A Ultrasensitive mouse insulin ELISA Crystal Chem, USA Cat#90080 Adiponectin ELISA Crystal Chem, USA Cat#80569 Leptin ELISA Crystal Chem, USA Cat#90030 Deposited data RNA-seq data This paper GSE210900 https://www.ncbi.nlm.nih. gov/geo/query/acc.cgi?acc=GSE210900 Raw data This paper Mendeley Data: http://www.doi.org/ 10.17632/yvhn4hn3b8.1 Original code This paper https://zenodo.org/records/6904028 Experimental models: Cell lines 3T3-L1 cells ATCC Cat#CL-173 TIRRfl/fl MEFs This paper N/A TIRRKO MEFs This paper N/A p53KO;TIRRKO MEFs This paper N/A Experimental models: Organisms/strains EIIaCre mice Jax stock #003724 CD19-Cre mice Jax stock #006785 Adp-Cre mice Jax stock #028020 (Continued on next page) Cell Reports 43, 114337, June 25, 2024 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Camk2a-Cre mice Jax stock #005359 p53KO mice Jax stock #002101 TIRRfl/fl mice This paper N/A TIRRKO mice This paper N/A p53HET;TIRRKO mice This paper N/A p53KO;TIRRKO mice This paper N/A AdipoQ-CreTG/WT;TIRRfl/fl mice This paper N/A Camk2a-CreTG/WT;TIRRfl/fl mice This paper N/A TIRRBcell KO mice This paper N/A Oligonucleotides See Table S2 for qPCR and ChIP-qPCR primers N/A N/A Software and algorithms Graphpad Prism Graphpad Software Inc., La Jolla, CA, USA https://www.graphpad.com Python, version 3.7.10 pandas, numpy, scipy, statsmodels, sklearn and umap libraries The Python Software Foundation (PSF) https://www.python.org ImajeJ NIH https://imagej.nih.gov/ij/
RNA Sequencing:Article Title: Endogenous p53 inhibitor TIRR dissociates systemic metabolic health from oncogenic activity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Abca1 Novus Biologicals Cat# NB400-105; RRID: AB_10000630 anti-p53 primary antibody Leica Cat#P53-CM5P-L RRID: AB_563933 anti-phospho-p53 (Ser15) antibody Cell Signaling Cat#9284 RRID: AB_331464 anti-acetyl-p53 (Lys379) antibody Cell Signaling Cat#2570 RRID: AB_823591 Anti-Tubulin Santa Cruz Biotechnology Cat# sc-9104 RRID: AB_2241191 antiCD40 (Clone HM40-3) Invitrogen Cat#14-0402-82 RRID: AB_467228 anti-B220 (PE Rat anti-mouse CD45R-B220 clone RA3-6B2 BD) Pharmingen Cat#561878 RRID: AB_10893353 anti-IgG1 (FITC Rat anti-mouse IgG1 BD) Pharmingen Cat#553443 RRID: AB_394862 Biological samples Human visceral adipose tissue biopsies This paper N/A Mouse tissues (perigonadal adipose tissue, liver, hypothalamus, spleen, quadriceps muscle) This paper N/A Chemicals, peptides, and recombinant proteins Standard chow PicoLab Mouse Diet 20 LabDiet Cat# 5058 HFD, 60% kcal fat Research Diets Inc. Cat#D12492 Dexa instrument Piximus GE N/A Lipofectamine-RNAiMax Thermo Fisher Scientific Cat#13778030 IL4 R&D systems Cat#404-ML-010 DMEM GIBCO Cat# 11965 3-isobutyl-1-methylxanthine (IBMX) Sigma-Aldrich Cat#I5879 dexamethasone Sigma-Aldrich Cat#D4902 rosiglitazone Sigma-Aldrich Cat#R2408 Opti-MEM Thermo Fisher Scientific Cat#31985070 Critical commercial assays Piccolo lipid panel plus Fisher Scientific Cat#07-P02-12A Ultrasensitive mouse insulin ELISA Crystal Chem, USA Cat#90080 Adiponectin ELISA Crystal Chem, USA Cat#80569 Leptin ELISA Crystal Chem, USA Cat#90030 Deposited data RNA-seq data This paper GSE210900 https://www.ncbi.nlm.nih. gov/geo/query/acc.cgi?acc=GSE210900 Raw data This paper Mendeley Data: http://www.doi.org/ 10.17632/yvhn4hn3b8.1 Original code This paper https://zenodo.org/records/6904028 Experimental models: Cell lines 3T3-L1 cells ATCC Cat#CL-173 TIRRfl/fl MEFs This paper N/A TIRRKO MEFs This paper N/A p53KO;TIRRKO MEFs This paper N/A Experimental models: Organisms/strains EIIaCre mice Jax stock #003724 CD19-Cre mice Jax stock #006785 Adp-Cre mice Jax stock #028020 (Continued on next page) Cell Reports 43, 114337, June 25, 2024 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Camk2a-Cre mice Jax stock #005359 p53KO mice Jax stock #002101 TIRRfl/fl mice This paper N/A TIRRKO mice This paper N/A p53HET;TIRRKO mice This paper N/A p53KO;TIRRKO mice This paper N/A AdipoQ-CreTG/WT;TIRRfl/fl mice This paper N/A Camk2a-CreTG/WT;TIRRfl/fl mice This paper N/A TIRRBcell KO mice This paper N/A Oligonucleotides See Table S2 for qPCR and ChIP-qPCR primers N/A N/A Software and algorithms Graphpad Prism Graphpad Software Inc., La Jolla, CA, USA https://www.graphpad.com Python, version 3.7.10 pandas, numpy, scipy, statsmodels, sklearn and umap libraries The Python Software Foundation (PSF) https://www.python.org ImajeJ NIH https://imagej.nih.gov/ij/
Immunofluorescence:
Western Blot:
Article Title: Dual functions of NPC1L1 for cholesterol transport: Cholesterol capture and regulation of inner-leaflet cholesterol accessibility
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GFP rabbit IgG fraction Thermo Fisher Scientific Cat# A11122; RRID: AB_221569 Anti-Niemann-Pick type C1 Like-1 NOVUS Biologicals Cat# NB400-128; RRID: AB_10000815 Na, K-ATPase antibody Cell Signaling Technology Cat# 3010; RRID: AB_2060983 Anti-rabbit IgG Cytiva Cat# NA934; RRID: AB_772206 Chemicals, peptides, and recombinant proteins Phosphatidylcholine Nacalai Tesque Cat# 27554–01 Oleic acid Nacalai Tesque Cat# 25629–75 Monoolein Nacalai Tesque Cat# 23408–12 NaOH solution Nacalai Tesque Cat# 94611–45 HCl solution Nacalai Tesque Cat# 37313–25 [3H]cholesterol (cholesterol, [1, 2, 3H(N)]-) Perkin Elmer Cat# NET139 Lipoprotein deficient serum (LPDS) from fetal calf Sigma-Aldrich – Lysophosphatidylcholine Santa Cruz Biotechnology Cat# SC-473611 Sodium taurocholate FUJIFILM Wako Cat# 197–10033 Ezetimibe Biosynth Carbosynth Cat# FE23215 Imidazole FUJIFILM Wako Cat# 095–00015 NaH2PO3 FUJIFILM Wako Cat# 197–09705 Na2HPO3 FUJIFILM Wako Cat# 197–02865 Lysozyme FUJIFILM Wako Cat# 123–06721 NaCl Nacalai Tesque Cat# 31320–05 Glycerol Nacalai Tesque Cat# 17018–83 Isopropyl-β-thiogalactopyranoside (IPTG) Nacalai Tesque Cat# 19742–81 IMAC Ni-charged resin Bio-Rad Cat# 156–0135 Protease inhibitor cocktail Roche Cat# 05056489001 CBB Stain One Nacalai Tesque Cat# 04543–51 Paraformaldehyde FUJIFILM Wako Cat# 163–20145 VECTASHIELD Mounting Medium Vector Laboratories Cat# H-1000 Sodium dodecyl sulfate (SDS) Nacalai Tesque Cat# 31606–75 Tris (hydroxymethyl) aminomethane Nacalai Tesque Cat# 35406–91 IGEPAL CA-630 Sigma-Aldrich Cat# 18896 Sodium deoxycholate FUJIFILM Wako Cat# 192–08312 Page Ruler Prestatined Protein Ladder Thermo Fisher Scientific Cat# 26616 ECL Prime Western Blotting Detection Reagent Cytiva Cat# RPN2232 Experimental models: Cell lines McA-RH7777 cells ATCC CRL-1601 MDA-MB-231 cells ATCC HTB-26 Oligonucleotides siRNA targeting sequence: NPC1L1 #1: sense: 5′-GAGGCCUUCUUAGAGGAAA-3′ antisense: 5′-UUUCCUUCUAAGAAGGCCUC-3′ This paper N/A (Continued on next page) iScience 29, 117625, October 16, 2026 15 .. McA-RH7777 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Nacalai Tesque) supplemented with 10% fetal bovine serum (FBS) (Cosmo Bio, Tokyo, Japan), 2 mM L-glutamine (Nacalai Tesque), 1% MEM Non-Essential Amino Acid solution (NEAA) (Thermo Fisher Scientific, MA, USA), and 100 units/mL penicillin streptomycin (PCSM) (Nacalai Tesque).
Ubiquitin Proteomics:
Protease Inhibitor:
Article Title: Dual functions of NPC1L1 for cholesterol transport: Cholesterol capture and regulation of inner-leaflet cholesterol accessibility
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GFP rabbit IgG fraction Thermo Fisher Scientific Cat# A11122; RRID: AB_221569 Anti-Niemann-Pick type C1 Like-1 NOVUS Biologicals Cat# NB400-128; RRID: AB_10000815 Na, K-ATPase antibody Cell Signaling Technology Cat# 3010; RRID: AB_2060983 Anti-rabbit IgG Cytiva Cat# NA934; RRID: AB_772206 Chemicals, peptides, and recombinant proteins Phosphatidylcholine Nacalai Tesque Cat# 27554–01 Oleic acid Nacalai Tesque Cat# 25629–75 Monoolein Nacalai Tesque Cat# 23408–12 NaOH solution Nacalai Tesque Cat# 94611–45 HCl solution Nacalai Tesque Cat# 37313–25 [3H]cholesterol (cholesterol, [1, 2, 3H(N)]-) Perkin Elmer Cat# NET139 Lipoprotein deficient serum (LPDS) from fetal calf Sigma-Aldrich – Lysophosphatidylcholine Santa Cruz Biotechnology Cat# SC-473611 Sodium taurocholate FUJIFILM Wako Cat# 197–10033 Ezetimibe Biosynth Carbosynth Cat# FE23215 Imidazole FUJIFILM Wako Cat# 095–00015 NaH2PO3 FUJIFILM Wako Cat# 197–09705 Na2HPO3 FUJIFILM Wako Cat# 197–02865 Lysozyme FUJIFILM Wako Cat# 123–06721 NaCl Nacalai Tesque Cat# 31320–05 Glycerol Nacalai Tesque Cat# 17018–83 Isopropyl-β-thiogalactopyranoside (IPTG) Nacalai Tesque Cat# 19742–81 IMAC Ni-charged resin Bio-Rad Cat# 156–0135 Protease inhibitor cocktail Roche Cat# 05056489001 CBB Stain One Nacalai Tesque Cat# 04543–51 Paraformaldehyde FUJIFILM Wako Cat# 163–20145 VECTASHIELD Mounting Medium Vector Laboratories Cat# H-1000 Sodium dodecyl sulfate (SDS) Nacalai Tesque Cat# 31606–75 Tris (hydroxymethyl) aminomethane Nacalai Tesque Cat# 35406–91 IGEPAL CA-630 Sigma-Aldrich Cat# 18896 Sodium deoxycholate FUJIFILM Wako Cat# 192–08312 Page Ruler Prestatined Protein Ladder Thermo Fisher Scientific Cat# 26616 ECL Prime Western Blotting Detection Reagent Cytiva Cat# RPN2232 Experimental models: Cell lines McA-RH7777 cells ATCC CRL-1601 MDA-MB-231 cells ATCC HTB-26 Oligonucleotides siRNA targeting sequence: NPC1L1 #1: sense: 5′-GAGGCCUUCUUAGAGGAAA-3′ antisense: 5′-UUUCCUUCUAAGAAGGCCUC-3′ This paper N/A (Continued on next page) iScience 29, 117625, October 16, 2026 15 .. McA-RH7777 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Nacalai Tesque) supplemented with 10% fetal bovine serum (FBS) (Cosmo Bio, Tokyo, Japan), 2 mM L-glutamine (Nacalai Tesque), 1% MEM Non-Essential Amino Acid solution (NEAA) (Thermo Fisher Scientific, MA, USA), and 100 units/mL penicillin streptomycin (PCSM) (Nacalai Tesque).
Mutagenesis:
SYBR Green Assay:
In Situ:
Clinical Proteomics:
AST Assay:
Cell-Signaling:Article Title: Dual functions of NPC1L1 for cholesterol transport: Cholesterol capture and regulation of inner-leaflet cholesterol accessibility
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GFP rabbit IgG fraction Thermo Fisher Scientific Cat# A11122; RRID: AB_221569 Anti-Niemann-Pick type C1 Like-1 NOVUS Biologicals Cat# NB400-128; RRID: AB_10000815 Na, K-ATPase antibody Cell Signaling Technology Cat# 3010; RRID: AB_2060983 Anti-rabbit IgG Cytiva Cat# NA934; RRID: AB_772206 Chemicals, peptides, and recombinant proteins Phosphatidylcholine Nacalai Tesque Cat# 27554–01 Oleic acid Nacalai Tesque Cat# 25629–75 Monoolein Nacalai Tesque Cat# 23408–12 NaOH solution Nacalai Tesque Cat# 94611–45 HCl solution Nacalai Tesque Cat# 37313–25 [3H]cholesterol (cholesterol, [1, 2, 3H(N)]-) Perkin Elmer Cat# NET139 Lipoprotein deficient serum (LPDS) from fetal calf Sigma-Aldrich – Lysophosphatidylcholine Santa Cruz Biotechnology Cat# SC-473611 Sodium taurocholate FUJIFILM Wako Cat# 197–10033 Ezetimibe Biosynth Carbosynth Cat# FE23215 Imidazole FUJIFILM Wako Cat# 095–00015 NaH2PO3 FUJIFILM Wako Cat# 197–09705 Na2HPO3 FUJIFILM Wako Cat# 197–02865 Lysozyme FUJIFILM Wako Cat# 123–06721 NaCl Nacalai Tesque Cat# 31320–05 Glycerol Nacalai Tesque Cat# 17018–83 Isopropyl-β-thiogalactopyranoside (IPTG) Nacalai Tesque Cat# 19742–81 IMAC Ni-charged resin Bio-Rad Cat# 156–0135 Protease inhibitor cocktail Roche Cat# 05056489001 CBB Stain One Nacalai Tesque Cat# 04543–51 Paraformaldehyde FUJIFILM Wako Cat# 163–20145 VECTASHIELD Mounting Medium Vector Laboratories Cat# H-1000 Sodium dodecyl sulfate (SDS) Nacalai Tesque Cat# 31606–75 Tris (hydroxymethyl) aminomethane Nacalai Tesque Cat# 35406–91 IGEPAL CA-630 Sigma-Aldrich Cat# 18896 Sodium deoxycholate FUJIFILM Wako Cat# 192–08312 Page Ruler Prestatined Protein Ladder Thermo Fisher Scientific Cat# 26616 ECL Prime Western Blotting Detection Reagent Cytiva Cat# RPN2232 Experimental models: Cell lines McA-RH7777 cells ATCC CRL-1601 MDA-MB-231 cells ATCC HTB-26 Oligonucleotides siRNA targeting sequence: NPC1L1 #1: sense: 5′-GAGGCCUUCUUAGAGGAAA-3′ antisense: 5′-UUUCCUUCUAAGAAGGCCUC-3′ This paper N/A (Continued on next page) iScience 29, 117625, October 16, 2026 15 .. McA-RH7777 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Nacalai Tesque) supplemented with 10% fetal bovine serum (FBS) (Cosmo Bio, Tokyo, Japan), 2 mM L-glutamine (Nacalai Tesque), 1% MEM Non-Essential Amino Acid solution (NEAA) (Thermo Fisher Scientific, MA, USA), and 100 units/mL penicillin streptomycin (PCSM) (Nacalai Tesque).
Staining:Article Title: Dual functions of NPC1L1 for cholesterol transport: Cholesterol capture and regulation of inner-leaflet cholesterol accessibility
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GFP rabbit IgG fraction Thermo Fisher Scientific Cat# A11122; RRID: AB_221569 Anti-Niemann-Pick type C1 Like-1 NOVUS Biologicals Cat# NB400-128; RRID: AB_10000815 Na, K-ATPase antibody Cell Signaling Technology Cat# 3010; RRID: AB_2060983 Anti-rabbit IgG Cytiva Cat# NA934; RRID: AB_772206 Chemicals, peptides, and recombinant proteins Phosphatidylcholine Nacalai Tesque Cat# 27554–01 Oleic acid Nacalai Tesque Cat# 25629–75 Monoolein Nacalai Tesque Cat# 23408–12 NaOH solution Nacalai Tesque Cat# 94611–45 HCl solution Nacalai Tesque Cat# 37313–25 [3H]cholesterol (cholesterol, [1, 2, 3H(N)]-) Perkin Elmer Cat# NET139 Lipoprotein deficient serum (LPDS) from fetal calf Sigma-Aldrich – Lysophosphatidylcholine Santa Cruz Biotechnology Cat# SC-473611 Sodium taurocholate FUJIFILM Wako Cat# 197–10033 Ezetimibe Biosynth Carbosynth Cat# FE23215 Imidazole FUJIFILM Wako Cat# 095–00015 NaH2PO3 FUJIFILM Wako Cat# 197–09705 Na2HPO3 FUJIFILM Wako Cat# 197–02865 Lysozyme FUJIFILM Wako Cat# 123–06721 NaCl Nacalai Tesque Cat# 31320–05 Glycerol Nacalai Tesque Cat# 17018–83 Isopropyl-β-thiogalactopyranoside (IPTG) Nacalai Tesque Cat# 19742–81 IMAC Ni-charged resin Bio-Rad Cat# 156–0135 Protease inhibitor cocktail Roche Cat# 05056489001 CBB Stain One Nacalai Tesque Cat# 04543–51 Paraformaldehyde FUJIFILM Wako Cat# 163–20145 VECTASHIELD Mounting Medium Vector Laboratories Cat# H-1000 Sodium dodecyl sulfate (SDS) Nacalai Tesque Cat# 31606–75 Tris (hydroxymethyl) aminomethane Nacalai Tesque Cat# 35406–91 IGEPAL CA-630 Sigma-Aldrich Cat# 18896 Sodium deoxycholate FUJIFILM Wako Cat# 192–08312 Page Ruler Prestatined Protein Ladder Thermo Fisher Scientific Cat# 26616 ECL Prime Western Blotting Detection Reagent Cytiva Cat# RPN2232 Experimental models: Cell lines McA-RH7777 cells ATCC CRL-1601 MDA-MB-231 cells ATCC HTB-26 Oligonucleotides siRNA targeting sequence: NPC1L1 #1: sense: 5′-GAGGCCUUCUUAGAGGAAA-3′ antisense: 5′-UUUCCUUCUAAGAAGGCCUC-3′ This paper N/A (Continued on next page) iScience 29, 117625, October 16, 2026 15 .. McA-RH7777 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Nacalai Tesque) supplemented with 10% fetal bovine serum (FBS) (Cosmo Bio, Tokyo, Japan), 2 mM L-glutamine (Nacalai Tesque), 1% MEM Non-Essential Amino Acid solution (NEAA) (Thermo Fisher Scientific, MA, USA), and 100 units/mL penicillin streptomycin (PCSM) (Nacalai Tesque).
Multiple Displacement Amplification:Article Title: Dual functions of NPC1L1 for cholesterol transport: Cholesterol capture and regulation of inner-leaflet cholesterol accessibility
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GFP rabbit IgG fraction Thermo Fisher Scientific Cat# A11122; RRID: AB_221569 Anti-Niemann-Pick type C1 Like-1 NOVUS Biologicals Cat# NB400-128; RRID: AB_10000815 Na, K-ATPase antibody Cell Signaling Technology Cat# 3010; RRID: AB_2060983 Anti-rabbit IgG Cytiva Cat# NA934; RRID: AB_772206 Chemicals, peptides, and recombinant proteins Phosphatidylcholine Nacalai Tesque Cat# 27554–01 Oleic acid Nacalai Tesque Cat# 25629–75 Monoolein Nacalai Tesque Cat# 23408–12 NaOH solution Nacalai Tesque Cat# 94611–45 HCl solution Nacalai Tesque Cat# 37313–25 [3H]cholesterol (cholesterol, [1, 2, 3H(N)]-) Perkin Elmer Cat# NET139 Lipoprotein deficient serum (LPDS) from fetal calf Sigma-Aldrich – Lysophosphatidylcholine Santa Cruz Biotechnology Cat# SC-473611 Sodium taurocholate FUJIFILM Wako Cat# 197–10033 Ezetimibe Biosynth Carbosynth Cat# FE23215 Imidazole FUJIFILM Wako Cat# 095–00015 NaH2PO3 FUJIFILM Wako Cat# 197–09705 Na2HPO3 FUJIFILM Wako Cat# 197–02865 Lysozyme FUJIFILM Wako Cat# 123–06721 NaCl Nacalai Tesque Cat# 31320–05 Glycerol Nacalai Tesque Cat# 17018–83 Isopropyl-β-thiogalactopyranoside (IPTG) Nacalai Tesque Cat# 19742–81 IMAC Ni-charged resin Bio-Rad Cat# 156–0135 Protease inhibitor cocktail Roche Cat# 05056489001 CBB Stain One Nacalai Tesque Cat# 04543–51 Paraformaldehyde FUJIFILM Wako Cat# 163–20145 VECTASHIELD Mounting Medium Vector Laboratories Cat# H-1000 Sodium dodecyl sulfate (SDS) Nacalai Tesque Cat# 31606–75 Tris (hydroxymethyl) aminomethane Nacalai Tesque Cat# 35406–91 IGEPAL CA-630 Sigma-Aldrich Cat# 18896 Sodium deoxycholate FUJIFILM Wako Cat# 192–08312 Page Ruler Prestatined Protein Ladder Thermo Fisher Scientific Cat# 26616 ECL Prime Western Blotting Detection Reagent Cytiva Cat# RPN2232 Experimental models: Cell lines McA-RH7777 cells ATCC CRL-1601 MDA-MB-231 cells ATCC HTB-26 Oligonucleotides siRNA targeting sequence: NPC1L1 #1: sense: 5′-GAGGCCUUCUUAGAGGAAA-3′ antisense: 5′-UUUCCUUCUAAGAAGGCCUC-3′ This paper N/A (Continued on next page) iScience 29, 117625, October 16, 2026 15 .. McA-RH7777 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Nacalai Tesque) supplemented with 10% fetal bovine serum (FBS) (Cosmo Bio, Tokyo, Japan), 2 mM L-glutamine (Nacalai Tesque), 1% MEM Non-Essential Amino Acid solution (NEAA) (Thermo Fisher Scientific, MA, USA), and 100 units/mL penicillin streptomycin (PCSM) (Nacalai Tesque).
Sequencing:Article Title: Dual functions of NPC1L1 for cholesterol transport: Cholesterol capture and regulation of inner-leaflet cholesterol accessibility
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GFP rabbit IgG fraction Thermo Fisher Scientific Cat# A11122; RRID: AB_221569 Anti-Niemann-Pick type C1 Like-1 NOVUS Biologicals Cat# NB400-128; RRID: AB_10000815 Na, K-ATPase antibody Cell Signaling Technology Cat# 3010; RRID: AB_2060983 Anti-rabbit IgG Cytiva Cat# NA934; RRID: AB_772206 Chemicals, peptides, and recombinant proteins Phosphatidylcholine Nacalai Tesque Cat# 27554–01 Oleic acid Nacalai Tesque Cat# 25629–75 Monoolein Nacalai Tesque Cat# 23408–12 NaOH solution Nacalai Tesque Cat# 94611–45 HCl solution Nacalai Tesque Cat# 37313–25 [3H]cholesterol (cholesterol, [1, 2, 3H(N)]-) Perkin Elmer Cat# NET139 Lipoprotein deficient serum (LPDS) from fetal calf Sigma-Aldrich – Lysophosphatidylcholine Santa Cruz Biotechnology Cat# SC-473611 Sodium taurocholate FUJIFILM Wako Cat# 197–10033 Ezetimibe Biosynth Carbosynth Cat# FE23215 Imidazole FUJIFILM Wako Cat# 095–00015 NaH2PO3 FUJIFILM Wako Cat# 197–09705 Na2HPO3 FUJIFILM Wako Cat# 197–02865 Lysozyme FUJIFILM Wako Cat# 123–06721 NaCl Nacalai Tesque Cat# 31320–05 Glycerol Nacalai Tesque Cat# 17018–83 Isopropyl-β-thiogalactopyranoside (IPTG) Nacalai Tesque Cat# 19742–81 IMAC Ni-charged resin Bio-Rad Cat# 156–0135 Protease inhibitor cocktail Roche Cat# 05056489001 CBB Stain One Nacalai Tesque Cat# 04543–51 Paraformaldehyde FUJIFILM Wako Cat# 163–20145 VECTASHIELD Mounting Medium Vector Laboratories Cat# H-1000 Sodium dodecyl sulfate (SDS) Nacalai Tesque Cat# 31606–75 Tris (hydroxymethyl) aminomethane Nacalai Tesque Cat# 35406–91 IGEPAL CA-630 Sigma-Aldrich Cat# 18896 Sodium deoxycholate FUJIFILM Wako Cat# 192–08312 Page Ruler Prestatined Protein Ladder Thermo Fisher Scientific Cat# 26616 ECL Prime Western Blotting Detection Reagent Cytiva Cat# RPN2232 Experimental models: Cell lines McA-RH7777 cells ATCC CRL-1601 MDA-MB-231 cells ATCC HTB-26 Oligonucleotides siRNA targeting sequence: NPC1L1 #1: sense: 5′-GAGGCCUUCUUAGAGGAAA-3′ antisense: 5′-UUUCCUUCUAAGAAGGCCUC-3′ This paper N/A (Continued on next page) iScience 29, 117625, October 16, 2026 15 .. McA-RH7777 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Nacalai Tesque) supplemented with 10% fetal bovine serum (FBS) (Cosmo Bio, Tokyo, Japan), 2 mM L-glutamine (Nacalai Tesque), 1% MEM Non-Essential Amino Acid solution (NEAA) (Thermo Fisher Scientific, MA, USA), and 100 units/mL penicillin streptomycin (PCSM) (Nacalai Tesque).
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